A Highly Sensitive SPE-Derivatization-UHPLC-MS Approach for Quantitative Profiling of Carotenoid-derived Dialdehydes from Vegetables

Jianing Mi; Kunpeng Jia; Aparna Balakrishna; Qitong Feng; Salim Al-Babili

doi:10.1021/acs.jafc.9b01749

A Highly Sensitive SPE-Derivatization-UHPLC-MS Approach for Quantitative Profiling of Carotenoid-derived Dialdehydes from Vegetables

Jianing Mi, Kunpeng Jia, Aparna Balakrishna, Qitong Feng, Salim Al-Babili

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Oxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs), besides the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles in plant development and carotenoid-metabolism. However, detection of DIALs in plants is challenging due to their instability, low-abundance and poor ionization efficiency in mass spectrometry. Here, we developed a solid phase extraction (SPE) and derivatization protocol coupled with ultra-high performance liquid chromatography−mass spectrometry (UHPLC-MS) for quantitative profiling of DIALs. Our method significantly enhances the sensitivity of DIALs detection with detection limit of 0.05 pg/mg dried food materials, allowing unambiguous profiling of 30 endogenous DIALs with C5 to C24 from vegetables. Our work provides a new and efficient approach for determining the content of DIALs from various complex matrices, paving the way for uncovering the functions of DIALs in human health and plant growth and development.

Original language	English (US)
Journal	Journal of Agricultural and Food Chemistry
DOIs	https://doi.org/10.1021/acs.jafc.9b01749
State	Published - May 10 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): CRG2017
Acknowledgements: We thank Dr. Magnus Rueping, KAUST, for providing the labeled DIAL standard. This work was supported by base line funding and a Competitive Research Grant (CRG2017) given to Salim Al-Babili by the King Abdullah University of Science and Technology (KAUST), and the Bill and Melinda Gates Foundation (Grant number: OPP1194472).

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10.1021/acs.jafc.9b01749

https://repository.kaust.edu.sa/bitstream/10754/652857/1/acs.jafc.9b01749.pdf

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@article{0658d407737b43d6b4abc0b7e213b238,

title = "A Highly Sensitive SPE-Derivatization-UHPLC-MS Approach for Quantitative Profiling of Carotenoid-derived Dialdehydes from Vegetables",

abstract = "Oxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs), besides the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles in plant development and carotenoid-metabolism. However, detection of DIALs in plants is challenging due to their instability, low-abundance and poor ionization efficiency in mass spectrometry. Here, we developed a solid phase extraction (SPE) and derivatization protocol coupled with ultra-high performance liquid chromatography−mass spectrometry (UHPLC-MS) for quantitative profiling of DIALs. Our method significantly enhances the sensitivity of DIALs detection with detection limit of 0.05 pg/mg dried food materials, allowing unambiguous profiling of 30 endogenous DIALs with C5 to C24 from vegetables. Our work provides a new and efficient approach for determining the content of DIALs from various complex matrices, paving the way for uncovering the functions of DIALs in human health and plant growth and development.",

author = "Jianing Mi and Kunpeng Jia and Aparna Balakrishna and Qitong Feng and Salim Al-Babili",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): CRG2017 Acknowledgements: We thank Dr. Magnus Rueping, KAUST, for providing the labeled DIAL standard. This work was supported by base line funding and a Competitive Research Grant (CRG2017) given to Salim Al-Babili by the King Abdullah University of Science and Technology (KAUST), and the Bill and Melinda Gates Foundation (Grant number: OPP1194472).",

year = "2019",

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doi = "10.1021/acs.jafc.9b01749",

language = "English (US)",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

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AU - Mi, Jianing

AU - Jia, Kunpeng

AU - Balakrishna, Aparna

AU - Feng, Qitong

AU - Al-Babili, Salim

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): CRG2017 Acknowledgements: We thank Dr. Magnus Rueping, KAUST, for providing the labeled DIAL standard. This work was supported by base line funding and a Competitive Research Grant (CRG2017) given to Salim Al-Babili by the King Abdullah University of Science and Technology (KAUST), and the Bill and Melinda Gates Foundation (Grant number: OPP1194472).

PY - 2019/5/10

Y1 - 2019/5/10

N2 - Oxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs), besides the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles in plant development and carotenoid-metabolism. However, detection of DIALs in plants is challenging due to their instability, low-abundance and poor ionization efficiency in mass spectrometry. Here, we developed a solid phase extraction (SPE) and derivatization protocol coupled with ultra-high performance liquid chromatography−mass spectrometry (UHPLC-MS) for quantitative profiling of DIALs. Our method significantly enhances the sensitivity of DIALs detection with detection limit of 0.05 pg/mg dried food materials, allowing unambiguous profiling of 30 endogenous DIALs with C5 to C24 from vegetables. Our work provides a new and efficient approach for determining the content of DIALs from various complex matrices, paving the way for uncovering the functions of DIALs in human health and plant growth and development.

AB - Oxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs), besides the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles in plant development and carotenoid-metabolism. However, detection of DIALs in plants is challenging due to their instability, low-abundance and poor ionization efficiency in mass spectrometry. Here, we developed a solid phase extraction (SPE) and derivatization protocol coupled with ultra-high performance liquid chromatography−mass spectrometry (UHPLC-MS) for quantitative profiling of DIALs. Our method significantly enhances the sensitivity of DIALs detection with detection limit of 0.05 pg/mg dried food materials, allowing unambiguous profiling of 30 endogenous DIALs with C5 to C24 from vegetables. Our work provides a new and efficient approach for determining the content of DIALs from various complex matrices, paving the way for uncovering the functions of DIALs in human health and plant growth and development.

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JF - Journal of Agricultural and Food Chemistry

ER -

A Highly Sensitive SPE-Derivatization-UHPLC-MS Approach for Quantitative Profiling of Carotenoid-derived Dialdehydes from Vegetables

Abstract

Bibliographical note

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