A high quality Arabidopsis transcriptome for accurate transcript-level analysis of alternative splicing

Runxuan Zhang, Cristiane P. G. Calixto, Yamile Marquez, Peter Venhuizen, Nikoleta A. Tzioutziou, Wenbin Guo, Mark Spensley, Juan Carlos Entizne, Dominika Lewandowska, Sara ten Have, Nicolas Frei dit Frey, Heribert Hirt, Allan B. James, Hugh G. Nimmo, Andrea Barta, Maria Kalyna, John W. S. Brown

Research output: Contribution to journalArticlepeer-review

182 Scopus citations

Abstract

Alternative splicing generates multiple transcript and protein isoforms from the same gene and thus is important in gene expression regulation. To date, RNA-sequencing (RNA-seq) is the standard method for quantifying changes in alternative splicing on a genome-wide scale. Understanding the current limitations of RNA-seq is crucial for reliable analysis and the lack of high quality, comprehensive transcriptomes for most species, including model organisms such as Arabidopsis, is a major constraint in accurate quantification of transcript isoforms. To address this, we designed a novel pipeline with stringent filters and assembled a comprehensive Reference Transcript Dataset for Arabidopsis (AtRTD2) containing 82,190 non-redundant transcripts from 34 212 genes. Extensive experimental validation showed that AtRTD2 and its modified version, AtRTD2-QUASI, for use in Quantification of Alternatively Spliced Isoforms, outperform other available transcriptomes in RNA-seq analysis. This strategy can be implemented in other species to build a pipeline for transcript-level expression and alternative splicing analyses.
Original languageEnglish (US)
Pages (from-to)5061-5073
Number of pages13
JournalNucleic Acids Research
Volume45
Issue number9
DOIs
StatePublished - Apr 11 2017

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: Biotechnology and Biological Sciences Research Council (BBSRC) [BB/K013661/1 and BB/K006568/1 to J.B.; BB/K006835/1 to H.N.]; Scottish Government Rural and Environment Science and Analytical Services division (RESAS) [to J.B. and R.Z.]; Austrian Science Fund (FWF) [P26333 to M.K., DK W1207 to A.B.]; LABEX Saclay Plant Sciences [to H.H.]; BBSRC EASTBIO PhD studentships (to N.T. and J.C.); European Alternative Splicing Network of Excellence (EURASNET) [LSHG-CT-2005-518238] for catalyzing important collaborations. Funding for open access charge: University of Dundee.

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