A fine match between the stereoselective ligands and membrane pore size for enhanced chiral separation

Honglei Wang, Yi Li, Tai Shung Chung*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


A D,L-tryptophan separation factor of 12-15 and D-tryptophan yield of >95% have been successfully achieved through using human serum albumin (HSA) as the stereoselective ligand in an affinity ultrafiltration (UF) system. The obtained separation factor in this work is even higher than the intrinsic value of 8.5 of HSA. This synergism may arise from the fact that a fine match between the regular crystalline structure of HSA molecules and suitable pore size of membranes makes some HSA molecules be retained within the membrane cross-section, thus offering a second-stage binding opportunity for L-tryptophan molecules. Therefore, a simultaneous enhancement in separation factor and D-tryptophan yield has been fulfilled in this work. The feasibility of HSA regeneration after D,L-tryptophan separation has also been demonstrated through a series of pH adjustment experiments. This study reveals the applicability of HSA in affinity UF systems for chiral separation due to economization of material costs.

Original languageEnglish (US)
Pages (from-to)2284-2291
Number of pages8
JournalAIChE Journal
Issue number9
StatePublished - Sep 2009
Externally publishedYes


  • Affinity ultrafiltration
  • Chiral separation
  • Membrane pore size
  • Serum albumin
  • Stereoselective ligand

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • General Chemical Engineering


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