A Disposable Passive Microfluidic Device for Cell Culturing.

Francesco Guzzi; Patrizio Candeloro; Maria Laura Coluccio; Costanza Maria Cristiani; Elvira Immacolata Parrotta; Luana Scaramuzzino; Stefania Scalise; Elisabetta Dattola; Maria Antonia D'Attimo; Giovanni Cuda; Ernesto Lamanna; Lucia Carmela Passacatini; Ennio Carbone; Ulrich Krühne; Enzo M. Di Fabrizio; Gerardo Perozziello

doi:10.3390/bios10030018

A Disposable Passive Microfluidic Device for Cell Culturing.

Francesco Guzzi, Patrizio Candeloro, Maria Laura Coluccio, Costanza Maria Cristiani, Elvira Immacolata Parrotta, Luana Scaramuzzino, Stefania Scalise, Elisabetta Dattola, Maria Antonia D'Attimo, Giovanni Cuda, Ernesto Lamanna, Lucia Carmela Passacatini, Ennio Carbone, Ulrich Krühne, Enzo M. Di Fabrizio, Gerardo Perozziello

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μl/ℎ - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 hours. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 hours.

Original language	English (US)
Pages (from-to)	18
Journal	Biosensors
Volume	10
Issue number	3
DOIs	https://doi.org/10.3390/bios10030018
State	Published - Mar 4 2020

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the Project for Young Researchers financed by the Italian Ministry of Health’s ‘High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices’ (CUP J65C13001350001, project No. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University ‘Magna Graecia’ of Catanzaro. This work is cofounded with the support of the European Commission, FESR FES 2014-2020 and of
the Calabria Region. The authors are the only parties responsible for this work and the European Commission and Calabria Region disclaim any responsibility for the use of the information contained here.

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https://repository.kaust.edu.sa/bitstream/10754/661933/1/biosensors-10-00018.pdf

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Guzzi, F., Candeloro, P., Coluccio, M. L., Cristiani, C. M., Parrotta, E. I., Scaramuzzino, L., Scalise, S., Dattola, E., D'Attimo, M. A., Cuda, G., Lamanna, E., Passacatini, L. C., Carbone, E., Krühne, U., Di Fabrizio, E. M., & Perozziello, G. (2020). A Disposable Passive Microfluidic Device for Cell Culturing. Biosensors, 10(3), 18. https://doi.org/10.3390/bios10030018

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title = "A Disposable Passive Microfluidic Device for Cell Culturing.",

abstract = "In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μl/ℎ - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 hours. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 hours.",

author = "Francesco Guzzi and Patrizio Candeloro and Coluccio, {Maria Laura} and Cristiani, {Costanza Maria} and Parrotta, {Elvira Immacolata} and Luana Scaramuzzino and Stefania Scalise and Elisabetta Dattola and D'Attimo, {Maria Antonia} and Giovanni Cuda and Ernesto Lamanna and Passacatini, {Lucia Carmela} and Ennio Carbone and Ulrich Kr{\"u}hne and {Di Fabrizio}, {Enzo M.} and Gerardo Perozziello",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the Project for Young Researchers financed by the Italian Ministry of Health{\textquoteright}s {\textquoteleft}High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices{\textquoteright} (CUP J65C13001350001, project No. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University {\textquoteleft}Magna Graecia{\textquoteright} of Catanzaro. This work is cofounded with the support of the European Commission, FESR FES 2014-2020 and of the Calabria Region. The authors are the only parties responsible for this work and the European Commission and Calabria Region disclaim any responsibility for the use of the information contained here.",

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AU - Guzzi, Francesco

AU - Candeloro, Patrizio

AU - Coluccio, Maria Laura

AU - Cristiani, Costanza Maria

AU - Parrotta, Elvira Immacolata

AU - Scaramuzzino, Luana

AU - Scalise, Stefania

AU - Dattola, Elisabetta

AU - D'Attimo, Maria Antonia

AU - Cuda, Giovanni

AU - Lamanna, Ernesto

AU - Passacatini, Lucia Carmela

AU - Carbone, Ennio

AU - Krühne, Ulrich

AU - Di Fabrizio, Enzo M.

AU - Perozziello, Gerardo

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the Project for Young Researchers financed by the Italian Ministry of Health’s ‘High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices’ (CUP J65C13001350001, project No. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University ‘Magna Graecia’ of Catanzaro. This work is cofounded with the support of the European Commission, FESR FES 2014-2020 and of the Calabria Region. The authors are the only parties responsible for this work and the European Commission and Calabria Region disclaim any responsibility for the use of the information contained here.

PY - 2020/3/4

Y1 - 2020/3/4

N2 - In this work, a disposable passive microfluidic device for cell culturing that does not require any additional/external pressure sources is introduced. By regulating the height of fluidic columns and the aperture and closure of the source wells, the device can provide different media and/or drug flows, thereby allowing different flow patterns with respect to time. The device is made of two Polymethylmethacrylate (PMMA) layers fabricated by micro-milling and solvent assisted bonding and allows us to ensure a flow rate of 18.6 μl/ℎ - 7%/day, due to a decrease of the fluid height while the liquid is driven from the reservoirs into the channels. Simulations and experiments were conducted to characterize flows and diffusion in the culture chamber. Melanoma tumor cells were used to test the device and carry out cell culturing experiments for 48 hours. Moreover, HeLa, Jurkat, A549 and HEK293T cell lines were cultivated successfully inside the microfluidic device for 72 hours.

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JO - Biosensors

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ER -

A Disposable Passive Microfluidic Device for Cell Culturing.

Abstract

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