A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome

Adam J. Lukaszewski; Adriana Alberti; Andrew Sharpe; Andrzej Kilian; Antonio Michele Stanca; Beat Keller; Bernardo J. Clavijo; Bernd Friebe; Bikram Gill; Brande Wulff; Brett Chapman; Burkhard Steuernagel; Catherine Feuillet; Claire Viseux; Curtis Pozniak; Daniel S. Rokhsar; Darrin Klassen; David Edwards; Eduard Akhunov; Etienne Paux; Françoise Alfama; Frédéric Choulet; Fuminori Kobayashi; Gary J. Muehlbauer; Hadi Quesneville; Hana Šimková; Hélène Rimbert; Heidrun Gundlach; Hikmet Budak; Hiroaki Sakai; Hirokazu Handa; Hiroyuki Kanamori; Jacqueline Batley; Jan Vrána; Jane Rogers; Jarmila Číhalíková; Jaroslav Doležel; Jarrod Chapman; Jesse A. Poland; Jianzhong Wu; Jitendra Khurana; Jonathan Wright; Kai Christian Bader; Kellye Eversole; Kerrie Barry; Kirsten McLay; Klaus F.X. Mayer; Kuldeep Singh; Leah Clissold; Lise Pingault; Loïc Couderc; Luigi Cattivelli; Manuel Spannagl; Marie Kubaláková; Mario Caccamo; Martin Mascher; Matthew Bellgard; Matthias Pfeifer; Matthias Zytnicki; Melanie Febrer; Michael Alaux; Mihaela M. Martis; Mikaël Loaec; Moreno Colaiacovo; Nagendra K. Singh; Natasha Glover; Nicolas Guilhot; Nils Stein; Odd Arne Olsen; P. Ron Maclachlan; Parveen Chhuneja; Patrick Wincker; Pierre Sourdille; Primetta Faccioli; Ricardo H. Ramirez-Gonzalez; Robbie Waugh; Romana Šperková; Ron Knox; Rudi Appels; Sapna Sharma; Sarah Ayling; Sebastien Praud; Shichen Wang; Sigbjørn Lien; Simen Rød Sandve; Takashi Matsumoto; Takashi R. Endo; Takeshi Itoh; Thomas Nussbaumer; Thomas Wicker; Tsuyoshi Tanaka; Uwe Scholz; Valérie Barbe; Véronique Jamilloux; Yasunari Ogihara; Zdeňka Dubská

doi:10.1126/science.1251788

A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome

Adam J. Lukaszewski, Adriana Alberti, Andrew Sharpe, Andrzej Kilian, Antonio Michele Stanca, Beat Keller, Bernardo J. Clavijo, Bernd Friebe, Bikram Gill, Brande Wulff, Brett Chapman, Burkhard Steuernagel, Catherine Feuillet, Claire Viseux, Curtis Pozniak, Daniel S. Rokhsar, Darrin Klassen, David Edwards, Eduard Akhunov, Etienne PauxFrançoise Alfama, Frédéric Choulet, Fuminori Kobayashi, Gary J. Muehlbauer, Hadi Quesneville, Hana Šimková, Hélène Rimbert, Heidrun Gundlach, Hikmet Budak, Hiroaki Sakai, Hirokazu Handa, Hiroyuki Kanamori, Jacqueline Batley, Jan Vrána, Jane Rogers, Jarmila Číhalíková, Jaroslav Doležel, Jarrod Chapman, Jesse A. Poland, Jianzhong Wu, Jitendra Khurana, Jonathan Wright, Kai Christian Bader, Kellye Eversole, Kerrie Barry, Kirsten McLay, Klaus F.X. Mayer, Kuldeep Singh, Leah Clissold, Lise Pingault, Loïc Couderc, Luigi Cattivelli, Manuel Spannagl, Marie Kubaláková, Mario Caccamo, Martin Mascher, Matthew Bellgard, Matthias Pfeifer, Matthias Zytnicki, Melanie Febrer, Michael Alaux, Mihaela M. Martis, Mikaël Loaec, Moreno Colaiacovo, Nagendra K. Singh, Natasha Glover, Nicolas Guilhot, Nils Stein, Odd Arne Olsen, P. Ron Maclachlan, Parveen Chhuneja, Patrick Wincker, Pierre Sourdille, Primetta Faccioli, Ricardo H. Ramirez-Gonzalez, Robbie Waugh, Romana Šperková, Ron Knox, Rudi Appels, Sapna Sharma, Sarah Ayling, Sebastien Praud, Shichen Wang, Sigbjørn Lien, Simen Rød Sandve, Takashi Matsumoto, Takashi R. Endo, Takeshi Itoh, Thomas Nussbaumer, Thomas Wicker, Tsuyoshi Tanaka, Uwe Scholz, Valérie Barbe, Véronique Jamilloux, Yasunari Ogihara, Zdeňka Dubská

Research output: Contribution to journal › Article › peer-review

1308 Scopus citations

Abstract

An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum) genome has been produced by sequencing isolated chromosome arms. We have annotated 124,201 gene loci distributed nearly evenly across the homeologous chromosomes and subgenomes. Comparative gene analysis of wheat subgenomes and extant diploid and tetraploid wheat relatives showed that high sequence similarity and structural conservation are retained, with limited gene loss, after polyploidization. However, across the genomes there was evidence of dynamic gene gain, loss, and duplication since the divergence of the wheat lineages. A high degree of transcriptional autonomy and no global dominance was found for the subgenomes. These insights into the genome biology of a polyploid crop provide a springboard for faster gene isolation, rapid genetic marker development, and precise breeding to meet the needs of increasing food demand worldwide.

Original language	English (US)
Journal	Science
Volume	345
Issue number	6194
DOIs	https://doi.org/10.1126/science.1251788
State	Published - Jan 1 2014
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2022-09-13

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10.1126/science.1251788

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Lukaszewski, A. J., Alberti, A., Sharpe, A., Kilian, A., Stanca, A. M., Keller, B., Clavijo, B. J., Friebe, B., Gill, B., Wulff, B., Chapman, B., Steuernagel, B., Feuillet, C., Viseux, C., Pozniak, C., Rokhsar, D. S., Klassen, D., Edwards, D., Akhunov, E., ... Dubská, Z. (2014). A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome. Science, 345(6194). https://doi.org/10.1126/science.1251788

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title = "A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome",

abstract = "An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum) genome has been produced by sequencing isolated chromosome arms. We have annotated 124,201 gene loci distributed nearly evenly across the homeologous chromosomes and subgenomes. Comparative gene analysis of wheat subgenomes and extant diploid and tetraploid wheat relatives showed that high sequence similarity and structural conservation are retained, with limited gene loss, after polyploidization. However, across the genomes there was evidence of dynamic gene gain, loss, and duplication since the divergence of the wheat lineages. A high degree of transcriptional autonomy and no global dominance was found for the subgenomes. These insights into the genome biology of a polyploid crop provide a springboard for faster gene isolation, rapid genetic marker development, and precise breeding to meet the needs of increasing food demand worldwide.",

author = "Lukaszewski, {Adam J.} and Adriana Alberti and Andrew Sharpe and Andrzej Kilian and Stanca, {Antonio Michele} and Beat Keller and Clavijo, {Bernardo J.} and Bernd Friebe and Bikram Gill and Brande Wulff and Brett Chapman and Burkhard Steuernagel and Catherine Feuillet and Claire Viseux and Curtis Pozniak and Rokhsar, {Daniel S.} and Darrin Klassen and David Edwards and Eduard Akhunov and Etienne Paux and Fran{\c c}oise Alfama and Fr{\'e}d{\'e}ric Choulet and Fuminori Kobayashi and Muehlbauer, {Gary J.} and Hadi Quesneville and Hana {\v S}imkov{\'a} and H{\'e}l{\`e}ne Rimbert and Heidrun Gundlach and Hikmet Budak and Hiroaki Sakai and Hirokazu Handa and Hiroyuki Kanamori and Jacqueline Batley and Jan Vr{\'a}na and Jane Rogers and Jarmila {\v C}{\'i}hal{\'i}kov{\'a} and Jaroslav Dole{\v z}el and Jarrod Chapman and Poland, {Jesse A.} and Jianzhong Wu and Jitendra Khurana and Jonathan Wright and Bader, {Kai Christian} and Kellye Eversole and Kerrie Barry and Kirsten McLay and Mayer, {Klaus F.X.} and Kuldeep Singh and Leah Clissold and Lise Pingault and Lo{\"i}c Couderc and Luigi Cattivelli and Manuel Spannagl and Marie Kubal{\'a}kov{\'a} and Mario Caccamo and Martin Mascher and Matthew Bellgard and Matthias Pfeifer and Matthias Zytnicki and Melanie Febrer and Michael Alaux and Martis, {Mihaela M.} and Mika{\"e}l Loaec and Moreno Colaiacovo and Singh, {Nagendra K.} and Natasha Glover and Nicolas Guilhot and Nils Stein and Olsen, {Odd Arne} and Maclachlan, {P. Ron} and Parveen Chhuneja and Patrick Wincker and Pierre Sourdille and Primetta Faccioli and Ramirez-Gonzalez, {Ricardo H.} and Robbie Waugh and Romana {\v S}perkov{\'a} and Ron Knox and Rudi Appels and Sapna Sharma and Sarah Ayling and Sebastien Praud and Shichen Wang and Sigbj{\o}rn Lien and Sandve, {Simen R{\o}d} and Takashi Matsumoto and Endo, {Takashi R.} and Takeshi Itoh and Thomas Nussbaumer and Thomas Wicker and Tsuyoshi Tanaka and Uwe Scholz and Val{\'e}rie Barbe and V{\'e}ronique Jamilloux and Yasunari Ogihara and Zde{\v n}ka Dubsk{\'a}",

note = "Generated from Scopus record by KAUST IRTS on 2022-09-13",

year = "2014",

month = jan,

day = "1",

doi = "10.1126/science.1251788",

language = "English (US)",

volume = "345",

journal = "Science",

issn = "1095-9203",

publisher = "AMER ASSOC ADVANCEMENT SCIENCE",

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Lukaszewski, AJ, Alberti, A, Sharpe, A, Kilian, A, Stanca, AM, Keller, B, Clavijo, BJ, Friebe, B, Gill, B, Wulff, B, Chapman, B, Steuernagel, B, Feuillet, C, Viseux, C, Pozniak, C, Rokhsar, DS, Klassen, D, Edwards, D, Akhunov, E, Paux, E, Alfama, F, Choulet, F, Kobayashi, F, Muehlbauer, GJ, Quesneville, H, Šimková, H, Rimbert, H, Gundlach, H, Budak, H, Sakai, H, Handa, H, Kanamori, H, Batley, J, Vrána, J, Rogers, J, Číhalíková, J, Doležel, J, Chapman, J, Poland, JA, Wu, J, Khurana, J, Wright, J, Bader, KC, Eversole, K, Barry, K, McLay, K, Mayer, KFX, Singh, K, Clissold, L, Pingault, L, Couderc, L, Cattivelli, L, Spannagl, M, Kubaláková, M, Caccamo, M, Mascher, M, Bellgard, M, Pfeifer, M, Zytnicki, M, Febrer, M, Alaux, M, Martis, MM, Loaec, M, Colaiacovo, M, Singh, NK, Glover, N, Guilhot, N, Stein, N, Olsen, OA, Maclachlan, PR, Chhuneja, P, Wincker, P, Sourdille, P, Faccioli, P, Ramirez-Gonzalez, RH, Waugh, R, Šperková, R, Knox, R, Appels, R, Sharma, S, Ayling, S, Praud, S, Wang, S, Lien, S, Sandve, SR, Matsumoto, T, Endo, TR, Itoh, T, Nussbaumer, T, Wicker, T, Tanaka, T, Scholz, U, Barbe, V, Jamilloux, V, Ogihara, Y & Dubská, Z 2014, 'A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome', Science, vol. 345, no. 6194. https://doi.org/10.1126/science.1251788

TY - JOUR

T1 - A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome

AU - Lukaszewski, Adam J.

AU - Alberti, Adriana

AU - Sharpe, Andrew

AU - Kilian, Andrzej

AU - Stanca, Antonio Michele

AU - Keller, Beat

AU - Clavijo, Bernardo J.

AU - Friebe, Bernd

AU - Gill, Bikram

AU - Wulff, Brande

AU - Chapman, Brett

AU - Steuernagel, Burkhard

AU - Feuillet, Catherine

AU - Viseux, Claire

AU - Pozniak, Curtis

AU - Rokhsar, Daniel S.

AU - Klassen, Darrin

AU - Edwards, David

AU - Akhunov, Eduard

AU - Paux, Etienne

AU - Alfama, Françoise

AU - Choulet, Frédéric

AU - Kobayashi, Fuminori

AU - Muehlbauer, Gary J.

AU - Quesneville, Hadi

AU - Šimková, Hana

AU - Rimbert, Hélène

AU - Gundlach, Heidrun

AU - Budak, Hikmet

AU - Sakai, Hiroaki

AU - Handa, Hirokazu

AU - Kanamori, Hiroyuki

AU - Batley, Jacqueline

AU - Vrána, Jan

AU - Rogers, Jane

AU - Číhalíková, Jarmila

AU - Doležel, Jaroslav

AU - Chapman, Jarrod

AU - Poland, Jesse A.

AU - Wu, Jianzhong

AU - Khurana, Jitendra

AU - Wright, Jonathan

AU - Bader, Kai Christian

AU - Eversole, Kellye

AU - Barry, Kerrie

AU - McLay, Kirsten

AU - Mayer, Klaus F.X.

AU - Singh, Kuldeep

AU - Clissold, Leah

AU - Pingault, Lise

AU - Couderc, Loïc

AU - Cattivelli, Luigi

AU - Spannagl, Manuel

AU - Kubaláková, Marie

AU - Caccamo, Mario

AU - Mascher, Martin

AU - Bellgard, Matthew

AU - Pfeifer, Matthias

AU - Zytnicki, Matthias

AU - Febrer, Melanie

AU - Alaux, Michael

AU - Martis, Mihaela M.

AU - Loaec, Mikaël

AU - Colaiacovo, Moreno

AU - Singh, Nagendra K.

AU - Glover, Natasha

AU - Guilhot, Nicolas

AU - Stein, Nils

AU - Olsen, Odd Arne

AU - Maclachlan, P. Ron

AU - Chhuneja, Parveen

AU - Wincker, Patrick

AU - Sourdille, Pierre

AU - Faccioli, Primetta

AU - Ramirez-Gonzalez, Ricardo H.

AU - Waugh, Robbie

AU - Šperková, Romana

AU - Knox, Ron

AU - Appels, Rudi

AU - Sharma, Sapna

AU - Ayling, Sarah

AU - Praud, Sebastien

AU - Wang, Shichen

AU - Lien, Sigbjørn

AU - Sandve, Simen Rød

AU - Matsumoto, Takashi

AU - Endo, Takashi R.

AU - Itoh, Takeshi

AU - Nussbaumer, Thomas

AU - Wicker, Thomas

AU - Tanaka, Tsuyoshi

AU - Scholz, Uwe

AU - Barbe, Valérie

AU - Jamilloux, Véronique

AU - Ogihara, Yasunari

AU - Dubská, Zdeňka

N1 - Generated from Scopus record by KAUST IRTS on 2022-09-13

PY - 2014/1/1

Y1 - 2014/1/1

N2 - An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum) genome has been produced by sequencing isolated chromosome arms. We have annotated 124,201 gene loci distributed nearly evenly across the homeologous chromosomes and subgenomes. Comparative gene analysis of wheat subgenomes and extant diploid and tetraploid wheat relatives showed that high sequence similarity and structural conservation are retained, with limited gene loss, after polyploidization. However, across the genomes there was evidence of dynamic gene gain, loss, and duplication since the divergence of the wheat lineages. A high degree of transcriptional autonomy and no global dominance was found for the subgenomes. These insights into the genome biology of a polyploid crop provide a springboard for faster gene isolation, rapid genetic marker development, and precise breeding to meet the needs of increasing food demand worldwide.

AB - An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum) genome has been produced by sequencing isolated chromosome arms. We have annotated 124,201 gene loci distributed nearly evenly across the homeologous chromosomes and subgenomes. Comparative gene analysis of wheat subgenomes and extant diploid and tetraploid wheat relatives showed that high sequence similarity and structural conservation are retained, with limited gene loss, after polyploidization. However, across the genomes there was evidence of dynamic gene gain, loss, and duplication since the divergence of the wheat lineages. A high degree of transcriptional autonomy and no global dominance was found for the subgenomes. These insights into the genome biology of a polyploid crop provide a springboard for faster gene isolation, rapid genetic marker development, and precise breeding to meet the needs of increasing food demand worldwide.

UR - https://www.science.org/doi/10.1126/science.1251788

UR - http://www.scopus.com/inward/record.url?scp=84904382612&partnerID=8YFLogxK

U2 - 10.1126/science.1251788

DO - 10.1126/science.1251788

M3 - Article

SN - 1095-9203

VL - 345

JO - Science

JF - Science

IS - 6194

ER -

A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome

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