TY - JOUR
T1 - A chimeric IDD4 repressor constitutively induces immunity in Arabidopsis via the modulation of salicylic- and jasmonic acid homeostasis
AU - Volz, Ronny
AU - Kim, SoonKap
AU - Mi, Jianing
AU - Mariappan, Kiruthiga
AU - Siodmak, Anna
AU - Al-Babili, Salim
AU - Hirt, Heribert
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): BAS/1/1062
Acknowledgements: Funding for the project was provided to HH by baseline funding project BAS/1/1062 of the King Abdullah University of Science and Technology.
PY - 2019/4/15
Y1 - 2019/4/15
N2 - INDETERMINATE DOMAIN (IDD)/BIRD proteins belong to a highly-conserved plant-specific group of transcription factors with dedicated functions in plant physiology and development. Here, we took advantage of the chimeric repressor gene-silencing technology (CRES-T, SRDX) to widen our view on the role of IDD4/IMPERIAL EAGLE and IDD family members in plant immunity. The hypomorphic idd4SRDX lines are compromised in growth and show a robust auto-immune phenotype. Hormonal measurements revealed the concomitant accumulation of salicylic acid and jasmonic acid suggesting that IDDs are involved in regulating the metabolism of these biotic-stress hormones. The analysis of immunity-pathways showed enhanced activation of immune MAP kinase-signaling pathways, the accumulation of hydrogen-peroxide and spontaneous programmed cell-death. The transcriptome of non-elicited idd4SRDX lines can be aligned to approximately 40% of differentially expressed genes (DEGs) in flg22-treated wild-type plants. The pattern of DEGs implies IDDs as pivotal repressors of flg22-dependent gene induction. Infection experiments showed the increased resistance of idd4SRDX lines to Pseudomonas syringae and Botrytis cinerea implying a function of IDDs in defense adaptation to hemibiotrophs and necrotrophs. Genome-wide IDD4 DNA binding studies (DAP-SEQ) combined with DEG analysis of idd4SRDX lines identified IDD4-regulated functional gene clusters that contribute to plant growth and development. In summary, we discovered that the expression of idd4SRDX activates a wide range of defense-related-traits opening up the possibility to apply idd4SRDX as a powerful tool to stimulate innate immunity in engineered crops.
AB - INDETERMINATE DOMAIN (IDD)/BIRD proteins belong to a highly-conserved plant-specific group of transcription factors with dedicated functions in plant physiology and development. Here, we took advantage of the chimeric repressor gene-silencing technology (CRES-T, SRDX) to widen our view on the role of IDD4/IMPERIAL EAGLE and IDD family members in plant immunity. The hypomorphic idd4SRDX lines are compromised in growth and show a robust auto-immune phenotype. Hormonal measurements revealed the concomitant accumulation of salicylic acid and jasmonic acid suggesting that IDDs are involved in regulating the metabolism of these biotic-stress hormones. The analysis of immunity-pathways showed enhanced activation of immune MAP kinase-signaling pathways, the accumulation of hydrogen-peroxide and spontaneous programmed cell-death. The transcriptome of non-elicited idd4SRDX lines can be aligned to approximately 40% of differentially expressed genes (DEGs) in flg22-treated wild-type plants. The pattern of DEGs implies IDDs as pivotal repressors of flg22-dependent gene induction. Infection experiments showed the increased resistance of idd4SRDX lines to Pseudomonas syringae and Botrytis cinerea implying a function of IDDs in defense adaptation to hemibiotrophs and necrotrophs. Genome-wide IDD4 DNA binding studies (DAP-SEQ) combined with DEG analysis of idd4SRDX lines identified IDD4-regulated functional gene clusters that contribute to plant growth and development. In summary, we discovered that the expression of idd4SRDX activates a wide range of defense-related-traits opening up the possibility to apply idd4SRDX as a powerful tool to stimulate innate immunity in engineered crops.
UR - http://hdl.handle.net/10754/631949
UR - https://academic.oup.com/pcp/advance-article/doi/10.1093/pcp/pcz057/5466459
UR - http://www.scopus.com/inward/record.url?scp=85069626840&partnerID=8YFLogxK
U2 - 10.1093/pcp/pcz057
DO - 10.1093/pcp/pcz057
M3 - Article
C2 - 30989238
SN - 0032-0781
VL - 60
SP - 1536
EP - 1555
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 7
ER -