A biocompatible oxidation-triggered carrier polymer with potential in therapeutics

Kyle E. Broaders; Sirisha Grandhe; Jean Frechet

doi:10.1021/ja110468v

A biocompatible oxidation-triggered carrier polymer with potential in therapeutics

Kyle E. Broaders, Sirisha Grandhe, Jean Frechet

Physical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

318 Scopus citations

Abstract

Dextran, a water-soluble, biocompatible polymer of glucose, was modified at its hydroxyls with arylboronic esters to make it soluble in common organic solvents, allowing for the facile preparation of oxidation-sensitive dextran (Oxi-DEX) carrier microparticles. These particles were found to release their payload with a half-life of 36 min at 1 mM H₂O₂, which can be compared with a half-life of greater than 1 week in the absence of H ₂O₂. When used in a model vaccine application, Oxi-DEX particles loaded with ovalbumin (OVA) increased the presentation to CD8 ⁺ T-cells 27-fold relative to OVA encapsulated in a classical vehicle not sensitive to oxidation. No presentation was observed from cells incubated with unencapsulated OVA Additionally, Oxi-DEX was found to be nontoxic in preliminary in vitro cytotoxicity assays. Because it is easy to prepare, sensitive to biological oxidation, and biocompatible, this material may represent an attractive new platform for selective delivery applications.

Original language	English (US)
Pages (from-to)	756-758
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	133
Issue number	4
DOIs	https://doi.org/10.1021/ja110468v
State	Published - Feb 2 2011

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/ja110468v

Cite this

@article{93d8a0f0cc454928afbb4ed253dbea60,

title = "A biocompatible oxidation-triggered carrier polymer with potential in therapeutics",

abstract = "Dextran, a water-soluble, biocompatible polymer of glucose, was modified at its hydroxyls with arylboronic esters to make it soluble in common organic solvents, allowing for the facile preparation of oxidation-sensitive dextran (Oxi-DEX) carrier microparticles. These particles were found to release their payload with a half-life of 36 min at 1 mM H2O2, which can be compared with a half-life of greater than 1 week in the absence of H 2O2. When used in a model vaccine application, Oxi-DEX particles loaded with ovalbumin (OVA) increased the presentation to CD8 + T-cells 27-fold relative to OVA encapsulated in a classical vehicle not sensitive to oxidation. No presentation was observed from cells incubated with unencapsulated OVA Additionally, Oxi-DEX was found to be nontoxic in preliminary in vitro cytotoxicity assays. Because it is easy to prepare, sensitive to biological oxidation, and biocompatible, this material may represent an attractive new platform for selective delivery applications.",

author = "Broaders, {Kyle E.} and Sirisha Grandhe and Jean Frechet",

year = "2011",

month = feb,

day = "2",

doi = "10.1021/ja110468v",

language = "English (US)",

volume = "133",

pages = "756--758",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "4",

}

TY - JOUR

T1 - A biocompatible oxidation-triggered carrier polymer with potential in therapeutics

AU - Broaders, Kyle E.

AU - Grandhe, Sirisha

AU - Frechet, Jean

PY - 2011/2/2

Y1 - 2011/2/2

N2 - Dextran, a water-soluble, biocompatible polymer of glucose, was modified at its hydroxyls with arylboronic esters to make it soluble in common organic solvents, allowing for the facile preparation of oxidation-sensitive dextran (Oxi-DEX) carrier microparticles. These particles were found to release their payload with a half-life of 36 min at 1 mM H2O2, which can be compared with a half-life of greater than 1 week in the absence of H 2O2. When used in a model vaccine application, Oxi-DEX particles loaded with ovalbumin (OVA) increased the presentation to CD8 + T-cells 27-fold relative to OVA encapsulated in a classical vehicle not sensitive to oxidation. No presentation was observed from cells incubated with unencapsulated OVA Additionally, Oxi-DEX was found to be nontoxic in preliminary in vitro cytotoxicity assays. Because it is easy to prepare, sensitive to biological oxidation, and biocompatible, this material may represent an attractive new platform for selective delivery applications.

AB - Dextran, a water-soluble, biocompatible polymer of glucose, was modified at its hydroxyls with arylboronic esters to make it soluble in common organic solvents, allowing for the facile preparation of oxidation-sensitive dextran (Oxi-DEX) carrier microparticles. These particles were found to release their payload with a half-life of 36 min at 1 mM H2O2, which can be compared with a half-life of greater than 1 week in the absence of H 2O2. When used in a model vaccine application, Oxi-DEX particles loaded with ovalbumin (OVA) increased the presentation to CD8 + T-cells 27-fold relative to OVA encapsulated in a classical vehicle not sensitive to oxidation. No presentation was observed from cells incubated with unencapsulated OVA Additionally, Oxi-DEX was found to be nontoxic in preliminary in vitro cytotoxicity assays. Because it is easy to prepare, sensitive to biological oxidation, and biocompatible, this material may represent an attractive new platform for selective delivery applications.

UR - http://www.scopus.com/inward/record.url?scp=79851470598&partnerID=8YFLogxK

U2 - 10.1021/ja110468v

DO - 10.1021/ja110468v

M3 - Article

C2 - 21171594

AN - SCOPUS:79851470598

SN - 0002-7863

VL - 133

SP - 756

EP - 758

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 4

ER -

A biocompatible oxidation-triggered carrier polymer with potential in therapeutics

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this