Abstract
Main conclusion: 13C-isotope feeding experiments demonstrate that the apocarotenoid 9-cis-β-apo-10ʹ-carotenal is the precursor of several strigolactones in rice, providing a direct, in planta evidence for its role in strigolactone biosynthesis. Abstract: Strigolactones (SLs) are plant hormone that regulates plant architecture and mediates rhizospheric communications. Previous in vitro studies using heterogously produced enzymes unraveled the conversion of all-trans-β-carotene via the intermediate 9-cis-β-apo-10ʹ-carotenal into the SL precursor carlactone. However, a direct evidence for the formation of SLs from 9-cis-β-apo-10ʹ-carotenal is still missing. To provide this evidence, we supplied rice seedlings with 13C-labeled 9-cis-β-apo-10ʹ-carotenal and analyzed their SLs by LC–MS. Our results show that 9-cis-β-apo-10ʹ-carotenal is the SL precursor in planta and reveal, for the first time, the application of labeled long-chain apocarotenoids as a promising approach to investigate apocarotenoid metabolism and the genesis of carotenoid-derived growth regulators and signaling molecules.
Original language | English (US) |
---|---|
Article number | 88 |
Journal | PLANTA |
Volume | 256 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2022 |
Bibliographical note
Funding Information:We sincerely thank the support from the members of KAUST Analytical Core Lab and the Bioactives lab. This work was supported by baseline funding given to S. A-B from King Abdullah University of Science and Technology (KAUST). We thank Dr. Abdel Gabbar Babiker for providing Striga hermonthica seeds.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Keywords
- C-isotope
- 9-cis-β-apo-10ʹ-carotenal
- LC–MS
- Rice
- Striga
- Strigolactones
ASJC Scopus subject areas
- Genetics
- Plant Science