3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy

Tristan Colomb; Etienne Cuche; Pia Dahlgren; Anca M. Mariana; Frederic Montfort; Christian D. Depeursinge; Pierre Marquet; Pierre J. Magistrettir

doi:10.1109/ISBI.2002.1029372

3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy

Tristan Colomb, Etienne Cuche, Pia Dahlgren, Anca M. Mariana, Frederic Montfort, Christian D. Depeursinge^*, Pierre Marquet, Pierre J. Magistrettir

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Scopus citations

Abstract

We report on a numerical method for the reconstruction of wavetonts in Digital Holographic Microscopy (DHM). It yields simultaneous amplitude and quantitative phase contrast imaging by acquiring a single hologram. The reconstruction method computes the propagation of the complex optical wavefront diffracted by the object and is used to determine the refractive index and/or shape of the object with accuracies in the nanometer range along the optical axis. The technique comprises the recording of a digital hologram of the specimen by means of a standard CCD camera at the exit of a Mach-Zender or Michelson type interferometer and an adjustment procedure to determine precisely the reconstruction parameters. The quantitative nature of the reconstructed phase distribution has been demonstrated by an application to surface profilometry where step height differences of a few nanometers have been measured. Another application takes place in biology for transmission phase-contrast imaging of living cells in culture with resolution reaching 30 nanometers in height.

Original language	English (US)
Title of host publication	2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings
Publisher	IEEE Computer Society
Pages	773-776
Number of pages	4
ISBN (Electronic)	078037584X
DOIs	https://doi.org/10.1109/ISBI.2002.1029372
State	Published - 2002
Externally published	Yes
Event	IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Washington, United States Duration: Jul 7 2002 → Jul 10 2002

Publication series

Name	Proceedings - International Symposium on Biomedical Imaging
Volume	2002-January
ISSN (Print)	1945-7928
ISSN (Electronic)	1945-8452

Other

Other	IEEE International Symposium on Biomedical Imaging, ISBI 2002
Country/Territory	United States
City	Washington
Period	07/7/02 → 07/10/02

Bibliographical note

Publisher Copyright:
© 2002 IEEE.

ASJC Scopus subject areas

Biomedical Engineering
Radiology Nuclear Medicine and imaging

Access to Document

10.1109/ISBI.2002.1029372

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Colomb, T., Cuche, E., Dahlgren, P., Mariana, A. M., Montfort, F., Depeursinge, C. D., Marquet, P., & Magistrettir, P. J. (2002). 3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy. In 2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings (pp. 773-776). Article 1029372 (Proceedings - International Symposium on Biomedical Imaging; Vol. 2002-January). IEEE Computer Society. https://doi.org/10.1109/ISBI.2002.1029372

Colomb, Tristan ; Cuche, Etienne ; Dahlgren, Pia et al. / 3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy. 2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings. IEEE Computer Society, 2002. pp. 773-776 (Proceedings - International Symposium on Biomedical Imaging).

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title = "3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy",

abstract = "We report on a numerical method for the reconstruction of wavetonts in Digital Holographic Microscopy (DHM). It yields simultaneous amplitude and quantitative phase contrast imaging by acquiring a single hologram. The reconstruction method computes the propagation of the complex optical wavefront diffracted by the object and is used to determine the refractive index and/or shape of the object with accuracies in the nanometer range along the optical axis. The technique comprises the recording of a digital hologram of the specimen by means of a standard CCD camera at the exit of a Mach-Zender or Michelson type interferometer and an adjustment procedure to determine precisely the reconstruction parameters. The quantitative nature of the reconstructed phase distribution has been demonstrated by an application to surface profilometry where step height differences of a few nanometers have been measured. Another application takes place in biology for transmission phase-contrast imaging of living cells in culture with resolution reaching 30 nanometers in height.",

author = "Tristan Colomb and Etienne Cuche and Pia Dahlgren and Mariana, {Anca M.} and Frederic Montfort and Depeursinge, {Christian D.} and Pierre Marquet and Magistrettir, {Pierre J.}",

note = "Publisher Copyright: {\textcopyright} 2002 IEEE.; IEEE International Symposium on Biomedical Imaging, ISBI 2002 ; Conference date: 07-07-2002 Through 10-07-2002",

year = "2002",

doi = "10.1109/ISBI.2002.1029372",

language = "English (US)",

series = "Proceedings - International Symposium on Biomedical Imaging",

publisher = "IEEE Computer Society",

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Colomb, T, Cuche, E, Dahlgren, P, Mariana, AM, Montfort, F, Depeursinge, CD, Marquet, P & Magistrettir, PJ 2002, 3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy. in 2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings., 1029372, Proceedings - International Symposium on Biomedical Imaging, vol. 2002-January, IEEE Computer Society, pp. 773-776, IEEE International Symposium on Biomedical Imaging, ISBI 2002, Washington, United States, 07/7/02. https://doi.org/10.1109/ISBI.2002.1029372

3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy. / Colomb, Tristan; Cuche, Etienne; Dahlgren, Pia et al.
2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings. IEEE Computer Society, 2002. p. 773-776 1029372 (Proceedings - International Symposium on Biomedical Imaging; Vol. 2002-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Colomb, Tristan

AU - Cuche, Etienne

AU - Dahlgren, Pia

AU - Mariana, Anca M.

AU - Montfort, Frederic

AU - Depeursinge, Christian D.

AU - Marquet, Pierre

AU - Magistrettir, Pierre J.

PY - 2002

Y1 - 2002

N2 - We report on a numerical method for the reconstruction of wavetonts in Digital Holographic Microscopy (DHM). It yields simultaneous amplitude and quantitative phase contrast imaging by acquiring a single hologram. The reconstruction method computes the propagation of the complex optical wavefront diffracted by the object and is used to determine the refractive index and/or shape of the object with accuracies in the nanometer range along the optical axis. The technique comprises the recording of a digital hologram of the specimen by means of a standard CCD camera at the exit of a Mach-Zender or Michelson type interferometer and an adjustment procedure to determine precisely the reconstruction parameters. The quantitative nature of the reconstructed phase distribution has been demonstrated by an application to surface profilometry where step height differences of a few nanometers have been measured. Another application takes place in biology for transmission phase-contrast imaging of living cells in culture with resolution reaching 30 nanometers in height.

AB - We report on a numerical method for the reconstruction of wavetonts in Digital Holographic Microscopy (DHM). It yields simultaneous amplitude and quantitative phase contrast imaging by acquiring a single hologram. The reconstruction method computes the propagation of the complex optical wavefront diffracted by the object and is used to determine the refractive index and/or shape of the object with accuracies in the nanometer range along the optical axis. The technique comprises the recording of a digital hologram of the specimen by means of a standard CCD camera at the exit of a Mach-Zender or Michelson type interferometer and an adjustment procedure to determine precisely the reconstruction parameters. The quantitative nature of the reconstructed phase distribution has been demonstrated by an application to surface profilometry where step height differences of a few nanometers have been measured. Another application takes place in biology for transmission phase-contrast imaging of living cells in culture with resolution reaching 30 nanometers in height.

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Colomb T, Cuche E, Dahlgren P, Mariana AM, Montfort F, Depeursinge CD et al. 3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy. In 2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings. IEEE Computer Society. 2002. p. 773-776. 1029372. (Proceedings - International Symposium on Biomedical Imaging). doi: 10.1109/ISBI.2002.1029372

3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy

Abstract

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Bibliographical note

ASJC Scopus subject areas

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