3D Concentric Electrodes-Based Alternating Current Electrohydrodynamics: Design, Simulation, Fabrication, and Potential Applications for Bioassays

Raphaela K.S. Silva; Sakandar Rauf; Ming Dong; Liang Chen; Hakan Bagci; Khaled N. Salama

doi:10.3390/bios12040215

3D Concentric Electrodes-Based Alternating Current Electrohydrodynamics: Design, Simulation, Fabrication, and Potential Applications for Bioassays

Raphaela K.S. Silva, Sakandar Rauf, Ming Dong, Liang Chen, Hakan Bagci, Khaled N. Salama^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Two-dimensional concentric asymmetric microelectrodes play a crucial role in developing sensitive and specific biological assays using fluid micromixing generated by alternating current electrohydrodynamics (ac-EHD). This paper reports the design, simulation, fabrication, and characterization of fluid motion generated by 3D concentric microelectrodes for the first time. Electric field simulations are used to compare electric field distribution at the electrodes and to analyze its effects on microfluidic micromixing in 2D and 3D electrodes. Three-dimensional devices show higher electric field peak values, resulting in better fluid micromixing than 2D devices. As a proof of concept, we design a simple biological assay comprising specific attachment of streptavidin beads onto the biotin-modified electrodes (2D and 3D), which shows ~40% higher efficiency of capturing specific beads in the case of 3D ac-EHD device compared to the 2D device. Our results show a significant contribution toward developing 3D ac-EHD devices that can be used to create more efficient biological assays in the future.

Original language	English (US)
Article number	215
Journal	Biosensors
Volume	12
Issue number	4
DOIs	https://doi.org/10.3390/bios12040215
State	Published - Apr 2022

Bibliographical note

Funding Information:
We acknowledge the financial support from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. K.N. Salama would like to acknowledge the funding from AMPM center under the CCF grant and the KAUST Sensor Initiative for supporting this work. H. Bagci would like to acknowledge the funding provided by KAUST Office of Sponsored Research (OSR) under Award 2019-CRG-4056.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

3D electrodes
alternating current electrohydrodynamics (ac-EHD)
bioassays
concentric electrodes
electric field simulation
fluid micromixing

ASJC Scopus subject areas

Analytical Chemistry
Biotechnology
Biomedical Engineering
Instrumentation
Engineering (miscellaneous)
Clinical Biochemistry

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10.3390/bios12040215

Cite this

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title = "3D Concentric Electrodes-Based Alternating Current Electrohydrodynamics: Design, Simulation, Fabrication, and Potential Applications for Bioassays",

abstract = "Two-dimensional concentric asymmetric microelectrodes play a crucial role in developing sensitive and specific biological assays using fluid micromixing generated by alternating current electrohydrodynamics (ac-EHD). This paper reports the design, simulation, fabrication, and characterization of fluid motion generated by 3D concentric microelectrodes for the first time. Electric field simulations are used to compare electric field distribution at the electrodes and to analyze its effects on microfluidic micromixing in 2D and 3D electrodes. Three-dimensional devices show higher electric field peak values, resulting in better fluid micromixing than 2D devices. As a proof of concept, we design a simple biological assay comprising specific attachment of streptavidin beads onto the biotin-modified electrodes (2D and 3D), which shows ~40% higher efficiency of capturing specific beads in the case of 3D ac-EHD device compared to the 2D device. Our results show a significant contribution toward developing 3D ac-EHD devices that can be used to create more efficient biological assays in the future.",

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note = "Funding Information: We acknowledge the financial support from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. K.N. Salama would like to acknowledge the funding from AMPM center under the CCF grant and the KAUST Sensor Initiative for supporting this work. H. Bagci would like to acknowledge the funding provided by KAUST Office of Sponsored Research (OSR) under Award 2019-CRG-4056. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Salama, Khaled N.

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N2 - Two-dimensional concentric asymmetric microelectrodes play a crucial role in developing sensitive and specific biological assays using fluid micromixing generated by alternating current electrohydrodynamics (ac-EHD). This paper reports the design, simulation, fabrication, and characterization of fluid motion generated by 3D concentric microelectrodes for the first time. Electric field simulations are used to compare electric field distribution at the electrodes and to analyze its effects on microfluidic micromixing in 2D and 3D electrodes. Three-dimensional devices show higher electric field peak values, resulting in better fluid micromixing than 2D devices. As a proof of concept, we design a simple biological assay comprising specific attachment of streptavidin beads onto the biotin-modified electrodes (2D and 3D), which shows ~40% higher efficiency of capturing specific beads in the case of 3D ac-EHD device compared to the 2D device. Our results show a significant contribution toward developing 3D ac-EHD devices that can be used to create more efficient biological assays in the future.

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3D Concentric Electrodes-Based Alternating Current Electrohydrodynamics: Design, Simulation, Fabrication, and Potential Applications for Bioassays

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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