TY - JOUR
T1 - 3D Analysis of Ordered Porous Polymeric Particles using Complementary Electron Microscopy Methods
AU - Alvarez, Juan
AU - Saudino, Giovanni
AU - Musteata, Valentina-Elena
AU - Madhavan, Poornima
AU - Genovese, Alessandro
AU - Behzad, Ali Reza
AU - Sougrat, Rachid
AU - Boi, Cristiana
AU - Peinemann, Klaus-Viktor
AU - Nunes, Suzana Pereira
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): BAS/1/1057-01-01
Acknowledgements: The work was supported by the King Abdullah University of Science and Technology (KAUST), base line funding BAS/1/1057-01-01. The authors thank LNLS (Brazil) for access to the SAXS1 beamline, particularly F. Meneau.
PY - 2019/9/27
Y1 - 2019/9/27
N2 - Highly porous particles with internal triply periodic minimal surfaces were investigated for sorption of proteins. The visualization of the complex ordered morphology requires complementary advanced methods of electron microscopy for 3D imaging, instead of a simple 2D projection: transmission electron microscopy (TEM) tomography, slice-and-view focused ion beam (FIB) and serial block face (SBF) scanning electron microscopy (SEM). The capability of each method of 3D image reconstruction was demonstrated and their potential of application to other synthetic polymeric systems was discussed. TEM has high resolution for details even smaller than 1 nm, but the imaged volume is relatively restricted (2.5 μm)3. The samples are pre-sliced in an ultramicrotome. FIB and SBF are coupled to a SEM. The sample sectioning is done in situ, respectively by an ion beam or an ultramicrotome, SBF, a method so far mostly applied only to biological systems, was particularly highly informative to reproduce the ordered morphology of block copolymer particles with 32-54 nm nanopores and sampling volume (20 μm)3.
AB - Highly porous particles with internal triply periodic minimal surfaces were investigated for sorption of proteins. The visualization of the complex ordered morphology requires complementary advanced methods of electron microscopy for 3D imaging, instead of a simple 2D projection: transmission electron microscopy (TEM) tomography, slice-and-view focused ion beam (FIB) and serial block face (SBF) scanning electron microscopy (SEM). The capability of each method of 3D image reconstruction was demonstrated and their potential of application to other synthetic polymeric systems was discussed. TEM has high resolution for details even smaller than 1 nm, but the imaged volume is relatively restricted (2.5 μm)3. The samples are pre-sliced in an ultramicrotome. FIB and SBF are coupled to a SEM. The sample sectioning is done in situ, respectively by an ion beam or an ultramicrotome, SBF, a method so far mostly applied only to biological systems, was particularly highly informative to reproduce the ordered morphology of block copolymer particles with 32-54 nm nanopores and sampling volume (20 μm)3.
UR - http://hdl.handle.net/10754/656929
UR - http://www.nature.com/articles/s41598-019-50338-2
UR - http://www.scopus.com/inward/record.url?scp=85072703964&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-50338-2
DO - 10.1038/s41598-019-50338-2
M3 - Article
C2 - 31562349
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
IS - 1
ER -