16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus

Daniele Daffonchio; Sara Borin; Arianna Consolandi; Diego Mora; Pier Luigi Manachini; Claudia Sorlini

doi:10.1016/S0378-1097(98)00168-2

16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus

Daniele Daffonchio^*, Sara Borin, Arianna Consolandi, Diego Mora, Pier Luigi Manachini, Claudia Sorlini

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The internal transcribed spacers between the 16S and the 23S ribosomal RNA genes were used to discriminate species of the 16S rRNA group 1 of the genus Bacillus by PCR. The spacer-PCR fingerprints clearly discriminated the different species, except those closely related like the members of the 'B. cereus group' (B. cereus, B. thuringiensis and B. mycoides) and the species of the 'B. subtilis group' (B. amyloliquefaciens and B. licheniformis). Examining in more detail the shortest internal transcribed spacers, B. subtilis group species were distinguished by single-strand conformation polymorphism analysis, whereas B. mycoides was differentiated from B. cereus/B. thuringiensis by restriction analysis.

Original language	English (US)
Pages (from-to)	229-236
Number of pages	8
Journal	FEMS Microbiology Letters
Volume	163
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(98)00168-2
State	Published - Jun 15 1998
Externally published	Yes

Keywords

16S rRNA group 1
Bacillus
Identification
Internal transcribed spacer- polymerase chain reaction fingerprinting
Restriction analysis
Single-strand conformation polymorphism

ASJC Scopus subject areas

Genetics
Molecular Biology
Microbiology

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10.1016/S0378-1097(98)00168-2

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title = "16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus",

abstract = "The internal transcribed spacers between the 16S and the 23S ribosomal RNA genes were used to discriminate species of the 16S rRNA group 1 of the genus Bacillus by PCR. The spacer-PCR fingerprints clearly discriminated the different species, except those closely related like the members of the 'B. cereus group' (B. cereus, B. thuringiensis and B. mycoides) and the species of the 'B. subtilis group' (B. amyloliquefaciens and B. licheniformis). Examining in more detail the shortest internal transcribed spacers, B. subtilis group species were distinguished by single-strand conformation polymorphism analysis, whereas B. mycoides was differentiated from B. cereus/B. thuringiensis by restriction analysis.",

keywords = "16S rRNA group 1, Bacillus, Identification, Internal transcribed spacer- polymerase chain reaction fingerprinting, Restriction analysis, Single-strand conformation polymorphism",

author = "Daniele Daffonchio and Sara Borin and Arianna Consolandi and Diego Mora and Manachini, {Pier Luigi} and Claudia Sorlini",

year = "1998",

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doi = "10.1016/S0378-1097(98)00168-2",

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T1 - 16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus

AU - Daffonchio, Daniele

AU - Borin, Sara

AU - Consolandi, Arianna

AU - Mora, Diego

AU - Manachini, Pier Luigi

AU - Sorlini, Claudia

PY - 1998/6/15

Y1 - 1998/6/15

N2 - The internal transcribed spacers between the 16S and the 23S ribosomal RNA genes were used to discriminate species of the 16S rRNA group 1 of the genus Bacillus by PCR. The spacer-PCR fingerprints clearly discriminated the different species, except those closely related like the members of the 'B. cereus group' (B. cereus, B. thuringiensis and B. mycoides) and the species of the 'B. subtilis group' (B. amyloliquefaciens and B. licheniformis). Examining in more detail the shortest internal transcribed spacers, B. subtilis group species were distinguished by single-strand conformation polymorphism analysis, whereas B. mycoides was differentiated from B. cereus/B. thuringiensis by restriction analysis.

AB - The internal transcribed spacers between the 16S and the 23S ribosomal RNA genes were used to discriminate species of the 16S rRNA group 1 of the genus Bacillus by PCR. The spacer-PCR fingerprints clearly discriminated the different species, except those closely related like the members of the 'B. cereus group' (B. cereus, B. thuringiensis and B. mycoides) and the species of the 'B. subtilis group' (B. amyloliquefaciens and B. licheniformis). Examining in more detail the shortest internal transcribed spacers, B. subtilis group species were distinguished by single-strand conformation polymorphism analysis, whereas B. mycoides was differentiated from B. cereus/B. thuringiensis by restriction analysis.

KW - 16S rRNA group 1

KW - Bacillus

KW - Identification

KW - Internal transcribed spacer- polymerase chain reaction fingerprinting

KW - Restriction analysis

KW - Single-strand conformation polymorphism

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16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus

Abstract

Keywords

ASJC Scopus subject areas

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