High-throughput sequencing system - Hiseq 4000

  • Ming Sin Cheung (Manager)

Equipment/facility: Equipment

  • LocationShow on map

    King Abdullah University of Science and Technology Thuwal 23955

    Saudi Arabia

Equipments Details


The HiSeq 4000 Systems leverage sequencing by synthesis (SBS) technology, the most widely adopted next-generation sequencing (NGS) technology in the industry, to ensure high data quality. In contrast to the other Illumina platforms, HiSeq 4000 Systems utilize innovative patterned flow cell technology, which offers an exceptional level of throughput for diverse sequencing applications. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system. Offering ultra-high throughput, the HiSeq 4000 Systems deliver > 750 Gb per flow cell in 3.5 days. With the highest daily throughput and lowest price per sample across multiple applications, running dual-flow cell generates > 400 Gb per day and up to 1.5 Tb per run. This power allows sequencing of more samples at greater depth, generating richer, more meaningful data in less time. Large studies can be completed faster, with less hands-on time and at a lower cost. The HiSeq 4000 system includes the following features: • Dramatically Increased Data Output and Throughput. Maximize efficiency through innovative patterned flow cell technology • Exceptional Data Quality. Leverage proven, industry-leading Illumina sequencing by synthesis chemistry • Unsurpassed Capacity. Sequence 12 genomes, 100 transcriptomes, or 96 exomes in fewer than 3.5 days Applications: Whole genome sequencing, mRNA sequencing, total RNA sequencing, small RNA sequencing, ampicon sequencing, chip sequencing, Rad sequencing Model: HiSeq 4000


NameHigh-throughput sequencing system - Hiseq 4000
ManufacturersIllumina, Inc.


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