ced2 mutant has reduced expression of NCED3 in response to osmotic stress (PEG, polyethylene glycol) treatments compared to the wild type. Other ABA biosynthesis genes are also greatly reduced in ced2 under osmotic stress. We used microarrays to detail the global programme of gene expression under osmotic stress treatment and identified distinct classes of up/down-regulated genes in the stress pathway. Overall design: Arabidopsis seedlings were selected at 14 days with 6hr PEG treatment for RNA extraction andhybridization to the Arabidopsis ATH1 Gene expression arrays (Agilent Technologies). From Northern data we have known some of well-known stress responsive gene were significantly changed. We sought to obtain whole gene expression to analysis which genes were affected in the osmotic stress pathway.
|Date made available
|Feb 20 2015