This course provides a comprehensive overview of bioimaging techniques including fundamental concepts and applications, which allow biology students to design imaging experiments for their own research. The course covers basic optics and spectroscopy, optical microscopy techniques, advanced fluorescence microscopy, and single-molecule imaging techniques. The course also introduces label-free optical imaging methods including Raman and infrared microscopy, and holographic microscopy. Introduction to advanced methods for manipulation of single cells and single molecules (optical and magnetic tweezers), and correlative light and electron microscopy (CLEM) will be also provided, together with some concept about the newest in-situ transmission electron microscopy (TEM) for biological applications