B 309 Advances in DNA Sequencing Tech. II



This course will cover the development of sequencing technologies starting from Maxam-Gilbert sequencing reaction – Capillary (Sanger) sequencing (Dye Termination) - Next (2nd) Generation Sequencing Technologies (Illumina GAIIx and HiSeq2000; Roche 454 GS FLX Titanium, Applied Biosystem SOLiD4/SOLiD5500) – Single Molecule (3rd Generation) Sequencing Technologies (PacBio, Oxford NanoPore) and others (Ion Torent etc.). Students will get the opportunity to go through the theoretical details of the different strategies of the genome-, transcriptome-, smRNA, Exome-, ChIP- methylome- and amplicon (metagenomics) sequencing; and hands on experience on library preparation, set up sequencing reactions and sequence run using Illumina platform especially.
Course period06/11/11 → …
Course level300